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1. Mascarell, L., Frey, J. R., Michel, F., Lefkovits, I., Truffa-Bachi, P. 2000 ; Increased protein synthesis after T cell activation in presence of cyclosporin A. Transplantation 70, 340 348. Dumont, F. J., Staruch, M. J., Fischer, P., DaSilva, C., Camacho, R. 1998 ; Inhibition of T cell activation by pharmacologic disruption of the MEK1 ERK MAP kinase or calcineurin signaling pathways results in differential modulation of cytokine production. J. Immunol. 160, 2579 2589. Szabo, S. J., Kim, S. T., Costa, G. L., Zhang, X., Fathman, C. G., Glimcher, L. H. 2000 ; A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell 100, 655 669. Szabo, S. J., Sullivan, B. M., Stemmann, C., Satoskar, A. R., Sleckman, B. P., Glimcher, L. H. 2002 ; Distinct effects of T-bet in TH1 lineage commitment and IFN- production in CD4 and CD8 T cells. Science 295, 338 342. Yang, J., Murphy, T. L., Ouyang, W., Murphy, K. M. 1999 ; Induction of interferon- production in Th1 CD4 T cells: evidence for two distinct pathways for promoter activation. Eur. J. Immunol. 29, 548 555. Ouyang, W., Jacobson, N. G., Bhattacharya, D., Gorham, J. D., Fenoglio, D., Sha, W. C., Murphy, T. L., Murphy, K. M. 1999 ; The Ets transcription factor ERM is Th1-specific and induced by IL-12 through a Stat4dependent pathway. Proc. Natl. Acad. Sci. USA 96, 3888 3893. Chan, S. H., Perussia, B., Gupta, J. W., Kobayashi, M., Pospisil, M., Young, H. A., Wolf, S. F., Young, D., Clark, S. C., Trinchieri, G. 1991 ; Induction of interferon production by natural killer cell stimulatory factor: characterization of the responder cells and synergy with other inducers. J. Exp. Med. 173, 869 879. Germann, T., Gately, M. K., Schoenhaut, D. S., Lohoff, M., Mattner, F., Fischer, S., Jin, S. C., Schmitt, E., Rude, E. 1993 ; Interleukin-12 T cell stimulating factor, a cytokine with multiple effects on T helper type 1 Th1 ; but not on Th2 cells. Eur. J. Immunol. 23, 17621770. 9. Kubin, M., Kamoun, M., Trinchieri, G. 1994 ; Interleukin 12 synergizes with B7 CD28 interaction in inducing efficient proliferation and cytokine production of human T cells. J. Exp. Med. 180, 211222. 10. Sica, A., Dorman, L., Viggiano, V., Cippitelli, M., Ghosh, P., Rice, N., Young, H. A. 1997 ; Interaction of NF- B and NFAT with the interferonpromoter. J. Biol. Chem. 272, 3041230420. 11. Kiani, A., Garcia-Cozar, F. J., Habermann, I., Laforsch, S., Aebischer, T., Ehninger, G., Rao, A. 2001 ; Regulation of interferon- gene expression by nuclear factor of activated T cells. Blood 98, 1480 1488. Campbell, P. M., Pimm, J., Ramassar, V., Halloran, P. F. 1996 ; Identification of a calcium-inducible, cyclosporine sensitive element in the IFN- promoter that is a potential NFAT binding site. Transplantation 61, 933939. 13. Peng, S. L., Gerth, A. J., Ranger, A. M., Glimcher, L. H. 2001 ; NFATc1 and NFATc2 together control both T and B cell activation and differentiation. Immunity 14, 1320. 14. Agarwal, S., Avni, O., Rao, A. 2000 ; Cell-type-restricted binding of the transcription factor NFAT to a distal IL-4 enhancer in vivo. Immunity 12, 643 652 and depakote.
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Results Plasma Concentrations of VPA Metabolites in Maternal, Fetal, and Newborn Sheep. A number of metabolites of VPA such as those formed via fatty acid -oxidation [ E ; -2-ene, E ; -3-ene, and 3-keto VPA], cytochrome P450-mediated desaturation 4-ene VPA ; , and hydroxylation and subsequent oxidation 3-OH, 4-OH, 5-OH, and 4-keto VPA, 2-PSA, 2-PGA ; were measured in maternal and fetal plasma during and after VPA administration Table 1 ; . Maternal and fetal plasma concentrations of 3-OH VPA and 2-PSA were generally below the lower limit of quantitation LOQ ; of the assay see footnote b in Table 1 for exceptions ; . Also, the 2-PGA metabolite was below the LOQ in all fetal plasma samples. During fetal infusion experiments, measurable concentrations of 2-PGA were detected in maternal plasma samples from only one animal. Concentrations of all VPA metabolites, except E ; -2-ene and 3-keto VPA, appeared to be relatively stable in maternal as well as in fetal plasma during the 20- to 24-h period of infusion and declined relatively rapidly to below LOQ levels within 12 to 24 postinfusion the figures of plasma profiles of these metabolites are not shown due to an apparent similarity between their shapes and time course ; . However, plasma concentrations of E ; -2-ene and 3-keto VPA were still increasing at the end of the infusion period and the maximal concentrations were typically observed a few hours later Fig. 2 ; . These two metabolites were detectable in maternal and fetal plasma for up to 36 after the end of infusion Fig. 2 ; . Table 1 presents the average maternal and fetal Cmax values of all metabolites during maternal and fetal administration experiments. Maternal plasma Cmax of VPA after maternal administration occurred at 5 min i.e., in the first sample after the i.v. bolus loading dose ; , whereas that after fetal administration occurred within 1 to 3 Similarly, the fetal plasma Cmax of VPA after fetal administration occurred at 5 min, whereas that after maternal administration occurred within 1 to 3 Maternal and fetal Cmax values of all metabolites, except E ; -2-ene and 3-keto VPA, occurred during the 20- to 24-h period of infusion; the Cmax values of these two metabolites occurred within 1 to 12 after the end of VPA infusion Fig. 2 ; . Plasma concentrations of VPA metabolites were also measured in samples obtained from four newborn lambs; samples could not be and diazepam and climara, for instance, climara estrogen patch.
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Received Jul. 25, 2002; revision accepted Jan. 21, 2003. For correspondence or reprints contact: Christopher R. Chitambar, MD, Division of Neoplastic Diseases, Medical College of Wisconsin, 9200 W. Wisconsin Ave., Milwaukee, WI 53226. E-mail: chitambr mcw.
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Assessing the Diabetic Foot for Signs of Neuropathy." American Academy of Family Physicians. Internet, January 2000. Black, Joan. "Diabetic Dilemma: Traditional or Western Medicine?" Windspeaker Contributor, Edmonton, Internet Wind-diabetes. 1998 Clinical Practice Guidelines for the Management of Diabetes in Canada. Supplement to CMAJ 1998, Canadian Diabetes Association, Canadian Medical Association Assessment, Nurse's Reference Library. Nursing 84 Books. Springhouse Corporation: Springhouse, Pennsylvania, 1984. Coggins, Kip. Alternative Pathways to Healing: The Recovery Medicine Wheel. Health Communications Inc., Florida, 1990. Definitions: Encyclopedia Dictionary, Nurse's Reference Library. Nursing 84 Book, Springhouse Corporation: Springhouse, Pennsylvania, 1984. "Diabetes and Aboriginal Peoples" Health Protections Branch Laboratory Center for Disease Control, Ottawa, Ontario. Internet, June, 1999. Elliot, Louise. "Cases of Diabetes Expected to Triple." Windspeaker Contributor, San Diego, California, Internet Wind-diabetes. "Feet Can Last A Lifetime: A Doctor's Guide to Early Identification of Foot Problems in Persons with Diabetes". MSN Health with MD. Internet, May 2001.
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